Pathological significance of abnormal recepteur d’origine nantais and programmed death ligand 1 expression in colorectal cancer

Figure 6 Expression of RON and PD-L1, and activation of signaling pathways in HT29 cells. A: HT29 cells were treated with 2 nmol/L MSP, 2 nmol/L MSP + 2 μmol/L BMS-777607, or 2 μmol/L BMS-777607 for 1 h. The proteins analyzed include RON, phosphorylated RON, AKT, ERK1/2, phosphorylated AKT, and phosphorylated-ERK1/2. GAPDH was used as a loading control; B-D: The expression of p-RON, p-AKT, and p-ERK1/2 was detected by Western blot and quantified. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.