Pathological significance of abnormal recepteur d’origine nantais and programmed death ligand 1 expression in colorectal cancer

Figure 5 Expression of RON and PD-L1 in HT29 cells after treatment with 2 nmol/L MSP, 2 nmol/L MSP + 2 μmol/L BMS-777607, or 2 μmol/L BMS-777607. A: Cellular immunofluorescence indicating the expression of RON and PD-L1 after treatment of HT29 cells with 2 nmol/L MSP, 2 nmol/L MSP + 2 μmol/L BMS-777607, or 2 μmol/L BMS-777607 for 24 h, respectively. DAPI indicates nuclei (blue color), FITC indicates PD-L1 (green color), and PE indicates RON (red color). Original magnification × 400 (all photomicrographs). B-C: HT29 cells were treated with 2 nmol/L MSP, 2 nmol/L MSP + 2 μmol/L BMS-777607, or 2 μmol/L BMS-777607 for 24 h, and the expression of RON and PD-L1 was detected by Western blot and quantified according to the immunoblots. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.