Pathological significance of abnormal recepteur d’origine nantais and programmed death ligand 1 expression in colorectal cancer

Figure 2 Four-color multiplex immunofluorescence staining of paraffin-embedded colorectal cancer tissues. A: High expression of RON and PD-L1 in tumor cells (TCs); B: High expression of RON in TCs and high expression of PD-L1 in tumor-infiltrating mononuclear cells (TIMCs); C: Lack of RON expression and high PD-L1 expression in TCs; D: Low expression of RON in TC and high PD-L1 expression in TIMCs; E: High RON expression in TCs and no PD-L1 expression in either TCs or TIMCs. Blue (DAPI) shows nuclei, green shows PD-L1, pink indicates RON, and red displays CK.