Pathological significance of abnormal recepteur d’origine nantais and programmed death ligand 1 expression in colorectal cancer

Table 4 Univariate and multivariate Cox proportional hazard analyses of overall survival in patients with colorectal cancer in the FAHZUSM and Gene Expression Omnibus cohorts

Variable	FAHZUSM cohort				GEO cohort
	Univariate analysis, HR (95%CI)	P value	Multivariate analysis, HR (95%CI)	P value	Univariate analysis, HR (95%CI)	P value	Multivariate analysis, HR (95%CI)	P value
Age (mean ± SD)	1.029 (1.011-1.048)	0.002	1.031 (1.011-1.050)	0.002	1.025 (1.009-1.041)	0.002	1.028 (1.012-1.045)	0.001
Gender
Male	1.000	0.011	1.000	0.008	1.000	0.293	1.000	0.076
Female	0.562 (0.362-0.874)		0.527 (0.328-0.847)		0.814 (0.555-1.194)		0.698 (0.469-1.039)
Principal diagnosis
Rectum	1.000	0.298	1.000	0.318	1.000	0.688	1.000	0.544
Colon	0.796 (0.518-1.224)		0.790 (0.497-1.255)		1.081 (0.738-1.585)		0.882 (0.589-1.322)
Histological type
Adenocarcinoma	1.000	0.408	1.000	0.387	NA
Mucinous/SRCC	2.464 (0.343-17.710)		2.720 (0.263-28.122)
Unknown	3.355 (0.433-25.986)		1.232 (0.129-11.795)
T stage
Tis-T2	1.000	< 0.001	1.000	0.003	1.000	0.001	1.000	0.007
T3	0.211 (0.108-0.413)		0.298 (0.141-0.632)		0.333 (0.145-0.766)		0.381 (0.159-0.914)
T4	0.503 (0.325-0.781)		0.596 (0.361-0.897)		0.455 (0.292-0.709)		0.476 (0.295-0.769)
N stage
N0	1.000	< 0.001	1.000	0.111	1.000	0.062	1.000	0.141
N (1-2)	3.156 (2.083-4.783)		5.376 (0.678-42.660)		1.435 (0.982-2.097)		0.306 (0.063-1.479)
M stage
M0	1.000	< 0.001	1.000	0.039	1.000	< 0.001	1.000	0.008
M1	4.338 (2.094-8.985)		2.445 (1.048-5.701)		3.546 (2.013-6.247)		2.481 (1.269-4.852)
Stage
0-II	1.000	< 0.001	1.000	0.406	1.000	0.029	1.000	0.099
III-IV	3.102 (2.044-4.708)		0.409 (0.050-3.366)		1.530 (1.045-2.242)		3.924 (0.774-19.888)
Pathological grade
Well/moderate	1.000	< 0.001	1.000	0.003	NA
Poor	7.689 (2.434-24.290)		4.044 (1.103-14.830)
Unknown	1.266 (0.513-3.126)		0.375 (0.104-1.357)
RON (protein)
Low	1.000	0.001	1.000	0.012	1.000	0.035	1.000	0.466
High	2.060 (1.364-3.112)		1.788 (1.138-2.808)		1.515 (1.030-2.228)		1.170 (0.767-1.785)
RON (mRNA)
Low	NA				1.000	0.035	1.000	0.466
High					1.515 (1.030-2.228)		1.170 (0.767-1.785)
PD-L1 (protein)
Low	1.000	< 0.001	1.000	0.001	NA
High (TIMCs)	0.544 (0.293-1.011)		0.676 (0.350-1.304)
High (TCs)	1.325 (0.669-2.511)		1.654 (0.819-3.338)
PD-L1 (mRNA)
Low	NA				1.000	0.047	1.000	0.083
High					1.835 (1.007-3.345)		1.719 (0.931-3.173)

All variables are adjusted by the Cox proportional hazard model. Bold entries indicate statistical significance (P < 0.05). GEO: The Gene Expression Omnibus; RON: Recepteur d’origine nantais; PD-L1: Programmed death ligand 1; TCs: Tumor cells; TIMCs: Tumor-infiltrating mononuclear cells; FAHZUSM: The First Affiliated Hospital, Zhejiang University School of Medicine.