Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways

Figure 6 Effect of QFGs on the regulation of PI3K/AKT and ERK signaling pathways in HCT-116 and HCT-8 cells. A, B: The expression and phosphorylation levels of PI3K, AKT and ERK in cells were determined by western blot. β-actin was used as an internal control. Each band is representative of triplicate experiments; C-H: The ratio of phosphorylation expression level/total expression level was quantitatively analyzed. ^aP < 0.05, ^bP < 0.01 vs the control group. QFGs: Qingjie Fuzheng granules; PI3K: Phosphatidylinositol 3-kinase; p‑PI3K, Phosphorylated‑PI3K; AKT: Protein kinase B; p‑AKT: Phosphorylated‑AKT; ERK: Extracellular regulated protein kinases.