Toll-like receptor 9 polymorphisms and Helicobacter pylori influence gene expression and risk of gastric carcinogenesis in the Brazilian population.

Table 1 Nucleotide primer sequences, polymerase chain reaction conditions and minor allele frequency for polymorphisms TLR9-1237 T/C (rs5743836) and -1486 T/C (rs187084)

Genes	Location	MAF	Primers, 5’-3’	Cycles	T melting	Enzyme	Genotypes, bp
TLR9-1237 T/C (rs5743836)	Chromosome 3: 52226766 (intron variant)	0.1725 (C)	F: TGGGAGCAGAGACATAATGGA	35	60 °C	BstNI	CC = 60 + 48 + 27
			R: CTGCTTGCAGTTGACTGTGT				CT = 108 + 60 + 48 + 27
							TT = 108 + 27
TLR9-1486 C/T (rs187084)	Chromosome 3: 52227015 (intron variant)	0.3776 (C)	F: TCCCAGCAGCAACAATTCATTA	30	60 °C	AflII	CC = 499
			R: CTGCTTGCAGTTGACTGTGT				TC = 499 + 327 + 172
							TT = 327 + 172

Minor allele frequency was extracted from 1000 Genomes Project Phase 3. MAF: Minor allele frequency.