MicroRNA-320a suppresses tumor progression by targeting PBX3 in gastric cancer and is downregulated by DNA methylation

Figure 4 Expression of miR-320a and methylation status of its promoter CpG islands in gastric cancer cell lines and tissue samples. A: Schematic of the location of miR-320a promoter CpG islands obtained from the human genome database and MethPrimer 2.0 (online); B: Methylation level of miR-320a in GES-1 and four gastric cancer (GC) cell lines; C: miR-320a expression and its promoter CpG island methylation level after 5-Aza-CdR and/or TSA treatment of MKN-45 cells; D: Representative MSP results of miR-320a promoter CPG islands in GC samples (cancer tissues and matched adjacent normal tissues). Cases with hypermethylation are marked. M: Methylated; U: Unmethylated; C: Cancer tissues; N: Normal tissues; *: Hypermethylation.