MicroRNA-320a suppresses tumor progression by targeting PBX3 in gastric cancer and is downregulated by DNA methylation

Figure 2 Overexpression of miR-320a inhibited gastric cancer cell viability and promoted apoptosis. A: Expression of miR-320a in gastric cancer (GC) cells was determined by quantitative PCR after transfection with miR-320a mimics (50 nmol/L), scramble mimics, miR-320a inhibitor (100 nmol/L), scramble inhibitor or no transfection; B: CCK8 assay was performed to determine cell viability after transfection at 0, 12, 24 and 72 h; C: Early apoptosis rate in the three groups is shown in the histogram; D: Flow cytometry was used to determine apoptosis after transfection for 48 h. The data are reported as means ± SD. ^aP < 0.05, ^bP < 0.01.