Copyright
©The Author(s) 2019.
World J Gastrointest Oncol. Oct 15, 2019; 11(10): 842-856
Published online Oct 15, 2019. doi: 10.4251/wjgo.v11.i10.842
Published online Oct 15, 2019. doi: 10.4251/wjgo.v11.i10.842
Name | Sequence (5’–3’) |
miR-320a reverse transcription primer | |
miR-320a | GTCTGTATGGTTGTTCTCGACTCCTTCACATCCCTATCCAACCATACAGACTCGCCCTC |
U6 snRNA | GGAACGCTTCACGAATTTG |
Real-time PCR primer sequence | |
miR-320a | FO:AAGGGATCGCGGGCG |
RE:TGCGTGTCGTGGAGTC | |
U6 snRNA | FO:ATTGGAACGATACAGAGAAGATT |
RE:GGAACGCTTCACGAATTTG | |
MSP primer sequence | |
Methylated | FO:ACGTCGTAATGTGAGGATTC |
RE:CGCAAACAAAACTCGATATAA | |
Nonmethylated | FO:ATTATGTTGTAATGTGAGGATTT |
RE:CACAAACAAAACTCAATATAACCC | |
miR-320a mimics sequence | |
miR-320a | Sense: AAAAGCUGGGUUGAGAGGGCGA |
Antisense: GCCCUCUCAACCCAGCUUUUUU | |
Negative control | Sense: UUCUUCGAACGUGUCACGUTT |
Antisense: ACGUGACACGUUCGGAGAATT | |
PBX3 primer sequence | FO:GCCAAATTGACCCAGATCAGAC |
RE:GAAATGGGACGTGTTCTACTCTGTT |
- Citation: Li YS, Zou Y, Dai DQ. MicroRNA-320a suppresses tumor progression by targeting PBX3 in gastric cancer and is downregulated by DNA methylation. World J Gastrointest Oncol 2019; 11(10): 842-856
- URL: https://www.wjgnet.com/1948-5204/full/v11/i10/842.htm
- DOI: https://dx.doi.org/10.4251/wjgo.v11.i10.842