MicroRNA-320a suppresses tumor progression by targeting PBX3 in gastric cancer and is downregulated by DNA methylation

doi:10.4251/wjgo.v11.i10.842

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastrointest Oncol. Oct 15, 2019; 11(10): 842-856
Published online Oct 15, 2019. doi: 10.4251/wjgo.v11.i10.842

Table 2 Primer/mimic sequences

Name	Sequence (5’–3’)
miR-320a reverse transcription primer
miR-320a	GTCTGTATGGTTGTTCTCGACTCCTTCACATCCCTATCCAACCATACAGACTCGCCCTC
U6 snRNA	GGAACGCTTCACGAATTTG
Real-time PCR primer sequence
miR-320a	FO:AAGGGATCGCGGGCG
miR-320a	RE:TGCGTGTCGTGGAGTC
U₆ snRNA	FO:ATTGGAACGATACAGAGAAGATT
U₆ snRNA	RE:GGAACGCTTCACGAATTTG
MSP primer sequence
Methylated	FO:ACGTCGTAATGTGAGGATTC
Methylated	RE:CGCAAACAAAACTCGATATAA
Nonmethylated	FO:ATTATGTTGTAATGTGAGGATTT
Nonmethylated	RE:CACAAACAAAACTCAATATAACCC
miR-320a mimics sequence
miR-320a	Sense: AAAAGCUGGGUUGAGAGGGCGA
miR-320a	Antisense: GCCCUCUCAACCCAGCUUUUUU
Negative control	Sense: UUCUUCGAACGUGUCACGUTT
Negative control	Antisense: ACGUGACACGUUCGGAGAATT
PBX3 primer sequence	FO:GCCAAATTGACCCAGATCAGAC
PBX3 primer sequence	RE:GAAATGGGACGTGTTCTACTCTGTT

PCR: Polymerase chain reaction.

Citation: Li YS, Zou Y, Dai DQ. MicroRNA-320a suppresses tumor progression by targeting PBX3 in gastric cancer and is downregulated by DNA methylation. World J Gastrointest Oncol 2019; 11(10): 842-856
URL: https://www.wjgnet.com/1948-5204/full/v11/i10/842.htm
DOI: https://dx.doi.org/10.4251/wjgo.v11.i10.842