Basic Study
Copyright ©The Author(s) 2019.
World J Gastrointest Oncol. Oct 15, 2019; 11(10): 842-856
Published online Oct 15, 2019. doi: 10.4251/wjgo.v11.i10.842
Table 2 Primer/mimic sequences
NameSequence (5’–3’)
miR-320a reverse transcription primer
miR-320aGTCTGTATGGTTGTTCTCGACTCCTTCACATCCCTATCCAACCATACAGACTCGCCCTC
U6 snRNAGGAACGCTTCACGAATTTG
Real-time PCR primer sequence
miR-320aFO:AAGGGATCGCGGGCG
RE:TGCGTGTCGTGGAGTC
U6 snRNAFO:ATTGGAACGATACAGAGAAGATT
RE:GGAACGCTTCACGAATTTG
MSP primer sequence
MethylatedFO:ACGTCGTAATGTGAGGATTC
RE:CGCAAACAAAACTCGATATAA
NonmethylatedFO:ATTATGTTGTAATGTGAGGATTT
RE:CACAAACAAAACTCAATATAACCC
miR-320a mimics sequence
miR-320aSense: AAAAGCUGGGUUGAGAGGGCGA
Antisense: GCCCUCUCAACCCAGCUUUUUU
Negative controlSense: UUCUUCGAACGUGUCACGUTT
Antisense: ACGUGACACGUUCGGAGAATT
PBX3 primer sequenceFO:GCCAAATTGACCCAGATCAGAC
RE:GAAATGGGACGTGTTCTACTCTGTT