Histological analysis of human pancreatic carcinoma following irreversible electroporation in a nude mouse model

Figure 3 Tumor tissue sections at 1 d and 7 d after the irreversible electroporation treatment were stained for Ki67 and cleaved caspase-3. A: A representative immunohistochemistry image in an untreated pancreatic parenchyma; B: In tumor tissues, extensive caspase-3 activation was observed on the first postoperative day. Irreversible electroporation significantly increased cell proliferation (Ki67 staining) at 1 d post-treatment, but cell proliferation was decreased at 7 d post-treatment (arrows); C: Limited caspase-3 staining at 7 d post-irreversible electroporation treatment was found in treated tumors, while most of the viable tumor tissues showed no caspase-3 activation. The slides were imaged at 400 × by light microscopy.