Histological analysis of human pancreatic carcinoma following irreversible electroporation in a nude mouse model

Figure 2 Hematoxylin and eosin staining of pancreatic cells in various groups. A: Histologically stained tissues of pancreatic parenchyma in untreated animals; B, C: Histology showed a normal pancreas after IRE, and a seepage area of erythrocytes was observed around the ablation zone (arrow). Additionally, larger amounts of erythrocytes were observed at 3 d post-IRE compared with 7 d post-IRE (black arrows represent the erythrocyte zone). The vascular structure was not damaged; D: Hematoxylin and eosin staining of tumor cells; E: Nuclear agglutination was observed 1 d post-IRE. The nucleus-to-cytoplasm ratio tended to increase; F: At 3 d post-therapy, a heterogeneous necrotizing tumor was present; G: Micrograph depicting the human pancreatic cancer cells 1 tumor xenograft 7 d post-IRE. A clear demarcation between the ablated (left side) and normal tumor (right side) tissues is depicted (arrows). Tumor cells were arranged more loosely in G compared with F (× 200 or × 400). IRE: Irreversible electroporation.