Chronic exposure to ethanol causes steatosis and inflammation in zebrafish liver

Figure 4 Effect of ethanol on mRNA liver expression of tumor necrosis factor-alpha and interleukin-1b. tnf-a was decreased significantly in E group compared to C at 2^nd week (P = 0.018) and increased along time up 4^th week (P < 0.001), reaching C group levels. Il1-b expression increased between 2 and 4 wk (P = 0.001) and at 4^th week there was a significant difference between C and E groups (P = 0.024). Statistical data were determined by the Kruskal-Wallis test and Dunn as post hoc test. Values significantly different where indicated: ^aSignificant statistical difference between 2 and 4 wk; ^bSignificant statistical difference between C and E groups. P < 0.05 was considered. tnf-a: Tumor necrosis factor-alpha; il: Interleukin.