Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2

Figure 4 Band of approximately 135 kD precipitated by mAb 1E4-1D9 was analyzed by LC-MS. A: Prediction of glycosylation sites and transmembrane region in hypothetical protein sequence gi60219551 was predicted by TMHMMM software; B: Number of cysteine was determined from all 1378 amino acid sequence, yellow highlight are N-glycosylation sites, green letter are cysteine, blue letter are glycosylation sites; C: Sequence of gi60219551 was aligned with glypican-3 based on the reliable program on website: https://blast.ncbi.nlm.nih.gov/Blast.cgi.