Characterization of a new monoclonal anti-glypican-3 antibody specific to the hepatocellular carcinoma cell line, HepG2

Figure 2 HepG2 cells (4 × 10⁵ cells/mL) were reacted with various final concentrations of mAb 1E4-1D9 (0-160 µg/mL) for 30 min on ice. Mouse IgG1 was used as isotype control. After washing, cells were stained with fluorescein isothiocyanate-conjugated rabbit anti-mouse Igs (1:20) for 30 min on ice and washed. Finally, cells were suspended with 300 μL of 0.5% paraformaldehyde in phosphate buffered saline, pH 7.2 and analyzed by flow cytometry.