Regulation of hepatic microRNA expression by hepatocyte nuclear factor 4 alpha

Figure 9 Effects of miR-194 and miR-192 on the activities of luciferase reporter vectors for the 3’UTR of mouse Chd1 and H3f3. Human hepatoma HepG2 cells were transfected with plasmid DNA including pmiR-Chd1 (or pmiR-H3f3), the pRL-CMV luciferase, and a synthetic mimic of miR-194/miR-192, or AllStars Negative Control siRNA (as negative control for microRNAs) using Lipofectamine 2000. Dual-luciferase reporter assay was conducted 24 h after transfection. The Y-axis represents relative luciferase activity for the 3’UTR of Chd1 or H3f3 normalized by the renilla luciferase. n = 4, Mean ± SE. ^aP < 0.05 compared to control (AllStars Negative Control siRNA). 3’UTR: Untranslated regions.