Regulation of hepatic microRNA expression by hepatocyte nuclear factor 4 alpha

Figure 8 Activation of (A) mouse miR-101b, (B) human miR-101-2, and (C) mouse miR-802 promoter by HNF4α. Human hepatoma HepG2 cells were transfected with firefly luciferase vectors containing microRNA promoter, pRL-CMV, and an expression vector for HNF4α and/or C/EBPα. Dual-luciferase reporter assay was conducted 24 h after transfection. The Y-axis represents relative luciferase activity for microRNA promoter normalized by the renilla luciferase. n = 4, Mean ± SE. ^aP < 0.05 compared to vector control; ^cP < 0.05 compared to HNF4α alone group. HNF4α: Hepatocyte nuclear factor 4 alpha; C/EBPα: CCAAT/enhancer-binding protein α.