Regulation of hepatic microRNA expression by hepatocyte nuclear factor 4 alpha

Figure 7 Activation of mouse (A) and human (B-D) miR-194-2/miR-192 promoter by HNF4α. Human hepatoma HepG2 cells were transfected with firefly luciferase vectors containing wild-type and mutant miR-194-2 promoter, pRL-CMV, and an expression vector for HNF4α/HNF1α. Dual-luciferase reporter assay was conducted 24 h after transfection. The y-axis represents relative luciferase activity for microRNA promoter normalized by the renilla luciferase. n = 4, Mean ± SE. ^aP < 0.05 compared to vector control; ^cP < 0.05 compared to HNF4α alone group. HNF4α: Hepatocyte nuclear factor 4 alpha.