Regulation of hepatic microRNA expression by hepatocyte nuclear factor 4 alpha

Figure 4 Analysis of DNAse-I hypersensitive sites as well as DNA-binding of HNF4α, RNA polymerase II (Pol2), and methylated histones to loci of miR-194-1/miR-215 (A), miR-26a-1 (B), miR-26a-2 (C) and miR-26b (D) in wildtype mouse liver. DNA-binding of HNF4α to these microRNA loci in the mouse small intestine (HNF4α _Int) was compared to those in the mouse liver (HNF4α_Liv). Data of DHSs (determined by DNAse-seq) and DNA-binding of proteins (determined by ChIP-seq) were retrieved from the public database of GEO DataSets and visualized in the IGV software. The peak values/ranges for each mark were shown in square brackets or under the line mark. DHSs: DNAse-I hypersensitive sites; H3K36me3: H3 trimethylation at lysine-36; H3K4me3: H3 trimethylation at lysine-4; H3K27me3: H3 trimethylation at lysine-27; H3K9me3: H3 trimethylation at lysine-9; Pol2: Polymerase 2; HNF4α: Hepatocyte nuclear factor 4 alpha; ChIP-seq: Chromatin immunoprecipitation-sequencing; IGV: Integrative genomics viewer.