Hepatitis C virus inhibitor synergism suggests multistep interactions between heat-shock protein 90 and hepatitis C virus replication

Figure 4 Radicicol and cyclosporin A have a synergistic effect on hepatitis C virus release into the medium. A: JFH1/HCVcc-infected Huh-7 cells were treated with 250 nmol/L CsA alone, 50 nmol/L radicicol alone, or both for 36 h. The total RNA was prepared from the Huh-7 cells, and the HCV RNA was quantified by reverse transcription-polymerase chain reaction (open columns, the scale on the left of the panel indicates the copies/well). The medium was replaced by the fresh medium containing the same levels of the drugs after 12 h, and total RNA was prepared from the medium 24 h later. The copy numbers of the HCV RNA in the medium are indicated by the filled columns (scale on the right of the panel); B: The cytotoxic effects of the drugs used in (A) on the Huh-7 cells carrying JFH1/HCVcc; C: The ratios of the RNA in the medium to the total RNA (as described above) are shown. HCV: Hepatitis C virus; HCVcc: Cell culture-derived HCV; CsA: Cyclosporin A.