Lack of hepcidin expression attenuates steatosis and causes fibrosis in the liver

Figure 5 Fibrosis in Hamp1 floxed or knockout mice fed high fat or regular diets for 3 mo. A: Fibrosis in the livers of floxed and knockout mice fed on regular or high fat sucrose (HFS) diets for 3 mo was detected by Sirius Red staining of tissue sections. Representative images obtained with Nikon Eclipse E400 light microscope are shown; B: 10 independent images (10 x) taken from each group were quantified using ImageJ ROI manager software. The collagen proportional area (CPA) was determined by calculating the percentage of collagen-occupied pixels against the total pixel values.