Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver

Figure 6 Liver microorgans histology. Hematoxylin-eosin. Samples were taken from control and preserved groups at the beginning (A, C and E) and at the end (t = 120 min) (B, D and F) of the experiment. Controls (A and B) showed normal hepatic parenchyma with fusiform endothelial cells (arrows) attached to perisinusoidal extracellular matrix and conserved hepatocyte cords. Sinusoids appeared dilated (bold arrow head) after 120 min. Liver microorgans (LMOs) preserved in BG35 (C and D) presented conserved hepatic architecture with fusiform (arrows) and rounded (bold arrows) endothelial cells. Sinusoids were dilated (bold arrow heads). LMOs preserved in ViaSpan^® (E and F) had fusiform (arrows) and rounded (bold arrows) endothelial cells, dilated sinusoids (bold arrow head), and balonized hepatocytes (b) at the beginning of the experiment. F: After 120 min, blebs (a) and areas of hepatocyte trabecular disruption (d) were also found. Central Vein (CV). Magnification × 200. n = 6 LMOs independent preparations for each condition. c: Central vein.