Interplay between microRNA-17-5p, insulin-like growth factor-II through binding protein-3 in hepatocellular carcinoma

Figure 2 Impact of microRNA-17-5p on insulin-like growth factor binding protein-3 mRNA expression in HuH-7 cell line. A: The expression of miR-17-5p was determined by TaqMan qRT-PCR in HuH-7 cells transfected with oligonucleotide mimics of miR-17-5p, 48 h post-transfection, relative to their expression in mock untransfected HuH-7 cells. The expression of miR-17-5p was normalized to RNU6B endogenous control. A: Transfection of miR-17-5p mimics increased miR-17-5p levels in HuH-7 by 250 fold compared to mock cells (P = 0.0470). Unpaired t-test was performed; B: HuH-7 cells were transfected with miR-17-5p mimics or inhibitors, and the relative expression of IGFBP-3 was determined using TaqMan qRT-PCR, relative to mock untransfected cells, and gene expression was normalized to endogenous control B2M. IGFBP-3 mRNA expression was dramatically suppressed upon mimicking of miR-17-5p compared to mock cells (P = 0.0267), while inhibitors of miR-17-5p showed a tendency of increase compared to mock cells. Unpaired t-test was performed. ^aP < 0.05. IGFBP-3: Insulin-like growth factor binding protein-3; miR-17-5p: MicroRNA-17-5p; qRT-PCR: Real-time quantitative PCR.