Anti-hepatitis C virus potency of a new autophagy inhibitor using human liver slices model

Figure 5 Inhibition of autophagy by treatment with GNS-396 (1 μmol/L) in primary adult human liver slices infected with cell culture-grown hepatitis C virus Con1 (multiplicity of infection = 1). A: Intracellular expression of LC3-I/LC3-II proteins in non-infected liver slices without treatment; B: Intracellular expression of LC3-I/LC3-II proteins and the normalization of intracellular protein LC3-II expression, for 10 d in non-infected liver slices with treatment either by GNS-396 (1 μmol/L) (G) or HCQ (1 μmol/L) (H) or without treatment (D: day); C: Expression of LC3-I/LC3-II proteins and HCV core and NS5A proteins in HCVcc Con1 infected liver slices either without treatment or (D) either with treatment by GNS-396 (1 μmol/L) or HCQ (1 μmol/L) and the normalization of Intracellular protein LC3-II expression for 10 d in HCVcc Con1 infected liver slices with treatment either by GNS-396 (1 μmol/L) (G) or HCQ (1 μmol/L) (H) or without treatment (D: day). LC3: Microtubule-associated protein 1A/1B-light chain; HCVcc: Cell culture-grown hepatitis C virus; NS5A: HCV nonstructural protein 5A.