Anti-hepatitis C virus potency of a new autophagy inhibitor using human liver slices model

Figure 3 Modulation and inhibition of autophagy by treatment with GNS-396 in HeLa cells. A and B: Autophagy modulation was evaluated using HeLa cells treated either with (A) GNS-396 or (B) HCQ at different concentrations (1, 10 and 100 μmol/L) during 6 h. HCQ was used as a positive control of autophagy modulation. Intracellular expression of proteins LC3 was evaluated by Western-blot assay and normalized for LC3-II; C and D: Autophagy inhibition by treatment with GNS-396 in HeLa cells. Autophagy inhibition was evaluated using HeLa cells treated either with (C) GNS-396 or (D) HCQ at different concentrations (1, 10 and 100 μmol/L) during 4 h in the presence or absence of 100 nmol/L bafilomycine A1. HCQ was used as a positive control of autophagy inhibition. LC3-II intracellular expression was evaluated by Western-blot assay and normalized. HCQ: Hydroxychloroquine; LC3: Microtubule-associated protein 1A/1B-light chain; DMEM: Dulbecco’s modified eagles’s medium; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; BafA1: Bafilomycin A1.