Obese diet-induced mouse models of nonalcoholic steatohepatitis-tracking disease by liver biopsy

Figure 5 Histological assessment of fibrosis stage and liver collagen at study end. Representative sirius red stained sections from; lean chow (A), ob/ob chow (B), DIO-NASH (C) and ob/ob NASH (D) mouse model. Liver fibrosis stage performed by a blinded pathologist at study end (E), and quantitatively image analysis of collagen (% area) from sirius red staining using visiomorph software (F). Fibrous band formation indicated by black arrows. ^aP < 0.05, ^bP < 0.01, ^dP < 0.001. The results are presented as mean ± SEM. Lean chow (n = 9), DIO-NASH (n = 12), ob/ob chow (n = 8), ob/ob NASH (n = 10). NASH: Nonalcoholic steatohepatitis; SEM: Standard error of the mean.