Obese diet-induced mouse models of nonalcoholic steatohepatitis-tracking disease by liver biopsy

Figure 4 Histological assessment of non-alcoholic fatty liver disease activity score and liver lipid at study end. Representative H and E stained sections from; lean chow (A), ob/ob chow (B), DIO-NASH (C) and ob/ob NASH (D) mouse models. NAFLD activity score (steatosis, inflammation and ballooning degeneration) performed by a blinded pathologist at study end (E), and quantitatively image analysis of steatosis (% area) from H and E staining using visiomorph software (F). Macrovesicular steatosis indicated by arrowheads, microvesicular steatosis indicated by short black arrows, inflammation indicated by short blue arrows, ballooning degeneration indicated by long black arrows. ^aP < 0.05, ^bP < 0.01, ^dP < 0.001. The results are presented as mean ± SEM. Lean chow (n = 9), DIO-NASH (n = 12), ob/ob chow (n = 8), ob/ob NASH (n = 10). NASH: Nonalcoholic steatohepatitis; NAFLD: Nonalcoholic fatty liver disease; SEM: Standard error of the mean.