Progress and prospects of engineered sequence-specific DNA modulating technologies for the management of liver diseases

Figure 4 Schematic representation of zinc finger nuclease interactions with DNA. Each zinc finger interacts with 3-4 bp in the major groove of the DNA double helix. The Hys2Cys2 side chains (depicted as the loop) bind to the DNA, which is facilitated by the interaction between the zinc finger protein (ZFP) and a Zinc ion (depicted as green circle) which stabilizes the protein structure. As well as FokI nuclease domains, ZFPs may also be bound to other effectors such as Krüppel-associated box or VP16/64.