Detection of hepatitis B virus infection: A systematic review

Table 2 Studies describing different quantitative molecular methods

Ref.	Year of publication	Method of quantitation	Detection limit
Garson et al[58]	2005	FRET based real time PCR assay	Sn at 95% detection level was 24.2 IU/mL
Welzel et al[59]	2006	Novel real time PCR	Sn at 95% detection level was 56 IU/mL
Mazet-Wagner et al[60]	2006	Real time PCR assay to detect total HBV DNA and cccDNA from serum and peripheral blood mononuclear cells	27 IU/mL
McCormick et al[61]	2006	Procleix Ultrio Assay (Multiplex PCR) to detect HIV 1, HCV RNA and HBV DNA simultaneously	Sp ≥ 99.5%, Sn is > 95% with detection limit for HBV DNA of 15 IU/mL
Cai et al[62]	2008	Real time fluorogenic Loop Mediated Isothermal Amplification (RtF-LAMP)	At 95% detection level 210 copies/mL
Paraskevis et al[63]	2010	New ultrasensitive in house real time PCR assay	Sn at 95% and 50% detection level: 22.2 IU/mL and 8.4 IU/mL
Chevaliez et al[64]	2010	v2.0 of the CAP/CTM assay	Highly Sn, could even detect 6.25 IU/mL HBV DNA; Sp is 99%, Intra-assay and interassay coefficients of variation ranged from 0.21% to 2.67% and from 0.65% to 2.25%, respectively
Sun et al[65]	2011	Duplex real-time PCR assay using two sets of primers/probes and a specific armored DNA as internal control	Detection limit 29.5 IU/mL; Sp 100%
Cha et al[66]	2013	ExiStation HBV diagnostic system	9.55 IU/mL
Yang et al[67]	2014	Colorimetric PCR with DNA zyme containing probe	Broad range of linearity and high sensitivity
Kania et al[68]	2014	2 in house real time PCR targeting X (qPCR1) or S (qPCR2) genes	qPCR1: 104 IU/mL; qPCR2: 91 IU/mL

FRET: Fluoresence resonant energy transfer; PCR: Polymerase chain reaction; cccDNA: Covalently closed circular DNA; Sn: Sensitivity; Sp: Specificity; HBV DNA: Hepatitis B virus deoxy ribonucleic acid; v2.0: Version 2.0; HCV: Hepatitis C virus; CAP/CTM: CobasAmpliPrep/CobasTaqMan.