Melatonin attenuates cisplatin-induced HepG2 cell death via the regulation of mTOR and ERCC1 expressions

Figure 6 Effects of melatonin and cisplatin on the cell cycle, as evaluated using flow cytometric analysis. Hepatocellular carcinoma (HepG2) cells were treated with melatonin (1 mmol/L) and/or cisplatin (20 μmol/L) for 24 h. Melatonin induced DNA accumulation in the HepG2 cells at the G₀/G₁ phase, whereas cisplatin affected cell cycle arrest at the sub-G₀, G₀/G₁, and S phases. In the combined treatment, melatonin decreased the cisplatin effect on cell cycle arrest at the sub-G₀ through G₀/G₁ phases but significantly increased S phase cell cycle arrest.