Melatonin attenuates cisplatin-induced HepG2 cell death via the regulation of mTOR and ERCC1 expressions

Figure 4 Histological changes. Hepatocellular carcinoma cells were treated with melatonin (1 mmol/L) and/or cisplatin (20 μmol/L) for 48 h. The cells were stained with DAPI for fragmented DNA (apoptotic bodies) and observed under an inverted microscope. A: Morphological changes showing vacuoles in the cytoplasm of cisplatin-treated cells (scale bar = 50 μm); B: DAPI staining showing nuclear condensation and apoptotic bodies (white arrows) in cisplatin-treated cells. The combined treatment showed less apoptotic effects (scale bar = 100 μm).