Copyright
©2014 Baishideng Publishing Group Inc.
World J Hepatol. Dec 27, 2014; 6(12): 916-922
Published online Dec 27, 2014. doi: 10.4254/wjh.v6.i12.916
Published online Dec 27, 2014. doi: 10.4254/wjh.v6.i12.916
Table 1 Characters of some isothermal amplification techniques[51]
NASBA | LAMP | SDA | RCA | HDA | RPA | |
Template | DNA, RNA | DNA1 | DNA1 | DNA1 | DNA1 | DNA1 |
No. of primers | 2 | 4-6 | 4 | 1 | 2 | 2 |
No. of enzymes | 3 | 1 | 2 | 2 | 2 | 2 |
Temperature (°C) | 41 | 60-65 | 37 | 37 | 65 | 30-42 |
Reaction duration (min) | 90-120 | 60-90 | 120 | 60 | 75-90 | 20 |
Denaturation step | Y | N | Y | N | N | N |
Inhibition tolerance | N | Y | N | N | Y | Y |
Product detection | GE, RT | GE, RT, TE | GE, RT | GE | GE, RT | RT |
Multiplex | Y | N | Y | N | Y | Y |
Point-of-care | Y | Y | Y | N | Y | Y |
- Citation: Zaghloul H, El-shahat M. Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis. World J Hepatol 2014; 6(12): 916-922
- URL: https://www.wjgnet.com/1948-5182/full/v6/i12/916.htm
- DOI: https://dx.doi.org/10.4254/wjh.v6.i12.916