Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis

Table 1 Characters of some isothermal amplification techniques[51]

	NASBA	LAMP	SDA	RCA	HDA	RPA
Template	DNA, RNA	DNA1	DNA1	DNA1	DNA1	DNA1
No. of primers	2	4-6	4	1	2	2
No. of enzymes	3	1	2	2	2	2
Temperature (°C)	41	60-65	37	37	65	30-42
Reaction duration (min)	90-120	60-90	120	60	75-90	20
Denaturation step	Y	N	Y	N	N	N
Inhibition tolerance	N	Y	N	N	Y	Y
Product detection	GE, RT	GE, RT, TE	GE, RT	GE	GE, RT	RT
Multiplex	Y	N	Y	N	Y	Y
Point-of-care	Y	Y	Y	N	Y	Y

¹RNA can be amplified after the introduction of a reverse transcription step. NASBA: Nucleic acid sequence-based amplification; LAMP: Loop-mediated isothermal amplification; SDA: Strand-displacement amplification; RCA: Rolling circle amplification; HDA: Helicase-dependent amplification; RPA: Recombinase polymerase amplification; GE: Gel Electrophoreses; RT: Real Time; TE: Turbidity; Y: Yes; N: No.