Role of autophagy in differential sensitivity of hepatocarcinoma cells to sorafenib

Figure 4 Gene silencing for AMPK. A: Huh7 cells were treated with siRNA for AMPK for 24 h. After 4 h treatment with SFN, LC3 was assessed with immunoblotting assay; B, C: The effect of either Met or SFN on hepatocellular carcinoma cell viability was determined by the MTT assay at 24 h in (B) Hep3B and (C) Huh7 cells. Some cells were treated with both agents. ^aP < 0.05, ^bP < 0.001. Met: Metformin; SFN: Sorafenib; CQ: Chloroquine.