Hypothermia predicts hepatic failure after extensive hepatectomy in mice

Figure 7 Actual findings of Western blot for p-STAT3, STAT3, p-AKT, AKT, p-ERK1/2,ERK1/2, p-SAPK/JNK, SAPK/JNK, p-p38, p38 and anti-glyceraldehyde-3-phosphate dehydrogenase. Using Western blot, signal transduction in the liver remnant at 24 and 48 h in hypothermic (HT) and normothermic (NT) mice was examined, with sham animals serving as controls. Although p-STAT3, p-AKT, p-ERK1/2 and p-p38 were up-regulated in the liver remnant after 75% partial hepatectomy in both HT and NT mice compared with the sham controls, there was no significant difference between the HT and NT groups. There was no change in p-SAPK/JNK. Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as an internal control.