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World J Hepatol. Mar 27, 2013; 5(3): 152-155
Published online Mar 27, 2013. doi: 10.4254/wjh.v5.i3.152
Published online Mar 27, 2013. doi: 10.4254/wjh.v5.i3.152
Figure 2 Electrophoresis with agarose gel 2%.
Reverse transcription polymerase chain reaction (RT-PCR) for hepatitis E virus (HEV) RNA from the liver specimen was performed using a First Strand cDNA synthesis kit for RT-PCR from Roche Diagnostics (Indianapolis, United States) according to the manufacturer’s instructions. This figure demonstrates the positive detection of HEV RNA with compatible strands 5 and 3. 1: Marker 100 bp (Promega); 2: White amplification; 3: Positive control for HEV RNA; 4: Negative control for HEV RNA; 5: Liver biopsy sample (HT370).
- Citation: Vieira CL, Baldaia C, Fatela N, Ramalho F, Cardoso C. Case of acute hepatitis E with concomitant signs of autoimmunity. World J Hepatol 2013; 5(3): 152-155
- URL: https://www.wjgnet.com/1948-5182/full/v5/i3/152.htm
- DOI: https://dx.doi.org/10.4254/wjh.v5.i3.152