Presence of disease specific autoantibodies against liver sinusoidal cells in primary biliary cirrhosis

Figure 1 Immunofluorescence of primary biliary cirrhosis sections incubated with primary biliary cirrhosis serum and fluorescein isothiocyanate -conjugated IgG or IgM secondary antibody. A-C: Negative control incubated with IgG secondary antibody; D-F: Primary biliary cirrhosis (PBC) section incubated with homologous PBC serum and IgG secondary antibody, showing staining of cholangiocytes, sinusoidal cells (weak staining) and mononuclear cells, probably lymphocytes; G-I: PBC section incubated with heterologous PBC serum and IgG secondary antibody, showing nuclear and cytoplasmic staining of hepatocytes and lymphocytes; J-L: PBC section incubated with homologous PBC serum and IgM secondary antibody, showing cytoplasmic staining of sinusoidal cells and lymphocytes. Magnification × 200. FITC: Fluorescein isothiocyanate.