Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH

Figure 3 Lipid quantification in different mouse models. A: Representative hematoxylin eosin (HE) staining of control (co) or methionine choline deficient diet (MCD) fed mice (× 200); B: Representative thin layer chromatography (TLC) detected with 2’,7’-dichlorofluorescein (DCF) (left) or sulfuric acid/ethanol (right); C: Quantification of TLC with ImageJ, detected with DCF (left) or sulfuric acid/ethanol (right). Results represent the mean ± SE from at least two independent TLC plates with n = 7 in each group; D: representative HE staining of wildtype (wt) and p62 transgenic mice (p62 tg)(× 200); E: Representative TLC detected with DCF (left) or sulfuric acid/ethanol (right); F: Quantification of TLC with ImageJ detected with DCF (left) or sulfuric acid/ethanol (right). Results represent the mean ± SE from at least two independent TLC plates and n = 4 in each group.