Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH

doi:10.4254/wjh.v5.i10.558

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Oct 27, 2013 (publication date) through Feb 18, 2025

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. Oct 27, 2013; 5(10): 558-567
Published online Oct 27, 2013. doi: 10.4254/wjh.v5.i10.558

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Figure 1 Quantification of lipids on the thin layer chromatography plate. A: Representative lipid dilution series (range: 2.5, 5, 7.5, 10, 12.5, 15, 20 μg) detected with 2’,7’-dichlorofluorescein (DCF) or sulfuric acid/ethanol; B: Quantification of the standard dilution series detected with DCF or sulfuric acid/ethanol and quantified with ImageJ. Results represent the mean ± SE from at least two independent thin layer chromatography plates. FFA: Free fatty acids.

Citation: Laggai S, Simon Y, Ranssweiler T, Kiemer AK, Kessler SM. Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH. World J Hepatol 2013; 5(10): 558-567
URL: https://www.wjgnet.com/1948-5182/full/v5/i10/558.htm
DOI: https://dx.doi.org/10.4254/wjh.v5.i10.558