Bone marrow progenitor cells do not contribute to liver fibrogenic cells

Figure 7 Representative analysis of CD38+ hepatic stellate cell subpopulations by flow cytometry. A: Two CD38+ subpopulations were observed: green fluorescent protein (GFP)+/CD38+ and GFP-/CD38+; B: Two subpopulations among GFP-/CD38+ cells were observed: GFP-/CD38+/CD45+ that identifies B lymphocytes, and GFP-/CD38+/CD45 that identifies hepatic stellate cells; C: Analysis of GFP+/CD38+ cells showed that all cells were of hematopoietic origin (CD45+); D: A significant increase of cells GFP/CD38+/CD45- was observed in liver tissue of the CCl₄ 30 d group when compared to the Saline 30 d group (Saline 30 d = 9.3% ± 2.4%; CCl₄ 30 d = 17.5% ± 3.9%, ^bP = 0.004). The statistical test used was Student’s t test.