Bone marrow progenitor cells do not contribute to liver fibrogenic cells

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Oct 27, 2012 (publication date) through Nov 8, 2024

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. Oct 27, 2012; 4(10): 274-283
Published online Oct 27, 2012. doi: 10.4254/wjh.v4.i10.274

Table 1 Antibody panel for flow cytometrric analysis of specific cells

Tubes	Cell number	Antibodies	Dil.	Target cells (tissue)
Control	1 x 10⁵	No antibodies	-	-
1	1 x 10⁶	CD45-PE-Cy7 (BD Biosciences) CD38-PE-Cy5 (eBioscience)	1:25 1:10	Hepatic stellate cells (liver)
2	1 x 10⁶	CD45-PE-Cy7 (BD Biosciences) CD14-APC (eBioscience) CD34-PE (eBioscience)	1:25 1:10 1:10	Hematopoietic stem cells, fibrocytes and monocytes (BM, PB and liver)
3	1 x 10⁶	CD45-PE-Cy7 (BD Biosciences) CD133-APC (eBioscience) CD34-PE (eBioscience)	1:25 1:10 1:10	Hematopoietic stem cells, fibrocytes and endothelial progenitor Cells (BM, PB and liver
4	1 x 10⁶	CD45-PE-Cy7 (BD Biosciences) CD117-APC (eBioscience) CD34-PE (eBioscience)	1:25 1:10 1:10	Common progenitor cells (BM)

BM: Bone marrow; PB: Peripheral blood.

Citation: Paredes BD, Faccioli LAP, Quintanilha LF, Asensi KD, Valle CZD, Canary PC, Takiya CM, Carvalho ACC, Goldenberg RCDS. Bone marrow progenitor cells do not contribute to liver fibrogenic cells. World J Hepatol 2012; 4(10): 274-283
URL: https://www.wjgnet.com/1948-5182/full/v4/i10/274.htm
DOI: https://dx.doi.org/10.4254/wjh.v4.i10.274