Nonmuscle myosin II regulates migration but not contraction in rat hepatic stellate cells

Figure 4 Nonmuscle myosin II isoform inhibition. A and B: Culture-activated hepatic stellate cells (Day 3) were incubated with nonmuscle myosin II-A or II-B, II-A & II-B, or scramble siRNA (100 nmol/L) for 48 h. RealTime PCR determined isoform-specific inhibition of each isoform as normalized to total cDNA concentration and compared to scramble control. C and D: In parallel experiments cell lysates were isolated and protein inhibition determined by western blot analysis. Protein expression was normalized to GAPDH and compared to scramble control (^aP < 0.05 as compared to scramble).