Nonmuscle myosin II regulates migration but not contraction in rat hepatic stellate cells

Figure 2 Immunocytochemical analysis of nonmuscle myosin II-A, II-B and II-C in hepatic stellate cells. Expression of all three isoforms was detected in culture-activated cells (Day 14). Specific II immunoreactivity of representative fields is shown in green; F-actin stress fibers (phalloidin) are shown in red and cell nuclei are stained blue (DAPI). Images of II isoforms, F-actin, and DAPI were taken separately at identical exposures and color channels were merged using IMAGE-PRO software (350 ×).