Construction of non-covalent single-chain Fv dimers for hepatocellular carcinoma and their biological functions

Figure 4 Immunohistochemistry of human hepatocellular carcinoma tissue. A: Reaction of scFv-3 dimer and human hepatocellular carcinoma tissue; B: Reaction of scFv-3 dimer and non- hepatocellular carcinoma tissue; C: Reaction of scFv-4 dimer and human hepatocellular carcinoma tissue; D: Reaction of scFv-4 dimer and non- hepatocellular carcinoma tissue; E: Reaction of scFv-5 dimer and human hepatocellular carcinoma tissue; F: Reaction of scFv-5 dimer and non- hepatocellular carcinoma tissue. Bound scFv dimers were detected with an antibody directed to the hexahistidine tag (Dianova) and peroxidase-conjugated rabbit anti-mouse immunoglobulins (Dako). Staining was performed with diaminobenzidine (DAB)/H₂O₂; nuclei were counterstained with hematoxylin. As expected, scFv dimers react with human hepatocellular carcinoma tissue but not with non- hepatocellular carcinoma tissue. The image was captured at 20 × magnification.