Construction of non-covalent single-chain Fv dimers for hepatocellular carcinoma and their biological functions

Figure 3 Analysis of soluble scFv dimers. A: Size exclusion chromatography of affinity purified scFv-3, scFv-4, scFv-5. Superimposed are elution profiles from a Superdex 200 gel filtration column of scFv-4 (solid line) and scFv-3 (dashed line). Elution peaks and molecular weight of calibration reference proteins are Gel Filtration Standard proteins (Biorad); B: Equilibrium binding curves of scFv dimers. Binding activity to hepatocellular carcinoma cell lines: ScFv-5 > scFv-4 > scFv-3 > HDM, with the tendency of decreasing binding activity going by diluted concentration. Determined By Model 680 Microplate Reader (Biorad).