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©2010 Baishideng.
World J Hepatol. Apr 27, 2010; 2(4): 151-161
Published online Apr 27, 2010. doi: 10.4254/wjh.v2.i4.151
Published online Apr 27, 2010. doi: 10.4254/wjh.v2.i4.151
Figure 2 Measurement of fucosylation-related tumor markers in gastrointestinal cancer.
A: The sera of patients with liver diseases were electrophoresed on an LCA agarose gel, followed by reaction with anti-AFP antibody. Since LCA specifically binds to fucosylated oligosaccharides on AFP, fucosylated AFP runs slowly on an LCA agarose gel; B: Since IgG has a fucosylated oligosaccharide in its Fc portion, a Fab fragment of anti-human haptoglobin IgG was coated on the bottom of a 96-well ELISA plate. After the sera of patients had been loaded into individual wells, the reaction with biotinylated AAL was performed to detect specifically fucosylated haptoglobin. Peroxidase-conjugated avidin and 3,3’,5,5’ tetramethylbenzisine were used for development.
- Citation: Moriwaki K, Miyoshi E. Fucosylation and gastrointestinal cancer. World J Hepatol 2010; 2(4): 151-161
- URL: https://www.wjgnet.com/1948-5182/full/v2/i4/151.htm
- DOI: https://dx.doi.org/10.4254/wjh.v2.i4.151