Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma

Table 2 Immunohistochemical reactivity for Cyclin D1 in all studied cases (mean ± SE) n(%)

Groups n = 125	Staining pattern	Positive expression	Immunohistochemical reactivity			Hepatic expression
			Mild (< 5%)	Moderate (5%-30%)	Marked (> 30%)
Control (n = 15)	Nuclear	3 (20.0)	3 (100)	0 (0)	0 (0)	1.33 ± 0.33
CHC without cirrhosis (n = 50)	Nuclear	33 (66.0)	25 (75.8)	8 (24.2)	0 (0)	6.60 ± 0.72a
CHC with cirrhosis (n = 30)	Nuclear	21 (70.0)	15 (71.4)	6 (28.6)	0 (0)	9.95 ± 1.70ab
HCV-related HCC (n = 30)	Nuclear	30 (100)	12 (40.0)	12 (40.0)	6 (20.0)	19.85 ± 3.90abc
Well-differentiated HCC (n = 15)	Nuclear	15 (100)	9 (60.0)	6 (40.0)	0 (0)	10.10 ± 2.20ab
Poorly-differentiated HCC (n = 15)	Nuclear	15 (100)	3 (20.0)	6 (40.0)	6 (40)	31.60 ± 6.20abcd

^aP < 0.001 vs controls;

^bP < 0.01 vs CHC without cirrhosis;

^cP < 0.001 vs LC;

^dP < 0.001 vs well differentiated HCC.