Mogroside V protects against acetaminophen-induced liver injury by reducing reactive oxygen species and c-jun-N-terminal kinase activation in mice

Figure 6 Mogroside V attenuates acetaminophen-induced hepatocytes damage in AML12 cells. AML 12 cells were treated with acetaminophen (APAP) (20 mM) supplemented with or without mogroside V (100 μM). A: Western blotting was used to detect the protein expression of p-c-jun-N-terminal kinase (JNK) and JNK after APAP exposure for six hours; B: Reactive oxygen species levels were detected 3 hours after APAP exposure; C: Cell death was measured by PI/Hoechst 33342 staining 24 hours after APAP exposure. Scale bar, 100 μm. MV: Mogroside V; APAP: Acetaminophen; GSH: Glutathione.