Yinchenhao decoction alleviates obstructive jaundice liver injury by modulating epidermal growth factor receptor and constitutive androstane receptor signaling

Figure 6 Yinchenhao decoction-induced regulation of constitutive androstane receptor expression and its nuclear heterotopy via epidermal growth factor receptor inhibition. A and B: Constitutive androstane receptor (CAR) expression and its nuclear heterotopia were assessed using immunofluorescence analysis in L02 cells (A) and HK-2 cells (B); C-F: Phosphorylated p-epidermal growth factor receptor and CAR levels of expression in L02 cells (C and D) and HK-2 cells (E and F). ^aP < 0.05 vs Sham (group S); ^bP < 0.01 vs group S; ^cP < 0.05 vs obstructive jaundice (OJ) model (Group O); ^dP < 0.01 vs group O; CAR: Constitutive androstane receptor; EGFR: Epidermal growth factor receptor; HY group: 5% OJ serum + 5% Yinchenhao decoction (YCHD)-containing serum + 5% FBS; HY + A group: 5% OJ + 5% YCHD-containing serum + 5% FBS + 10 μM NSC228155; Group O: Obstructive jaundice model.