Uncovering immune cell heterogeneity in hepatocellular carcinoma by combining single-cell RNA sequencing with T-cell receptor sequencing

Figure 3 Analysis of the development trajectories of the CD8⁺ T and CD4⁺ T-cell subsets. A: Schematic diagram of the pseudotime trajectory of CD8⁺ T-cell subpopulations at P0301 (left) and P0302 (right). Each dot corresponds to a single cell, and each color represents a T-cell cluster; B: The same trajectory analysis was applied to CD4⁺ T cells; C: Heatmap of differential gene expression using branch point expression analysis comparing different cell fates of CD8⁺ T cells at P0301. Each row represents a gene, and the column represents a total of 100 bins that divide the pseudotime value from start to finish; D: Branch point expression analysis of the same CD4⁺ T cells in P0301.