Uncovering immune cell heterogeneity in hepatocellular carcinoma by combining single-cell RNA sequencing with T-cell receptor sequencing

Figure 1 Single-cell transcriptome clustering of all cells from hepatocellular carcinoma patients. A: Schematic diagram of the single-cell RNA sequencing experimental design; B: Uniform manifold approximation and projections of the combined single-cell RNA sequencing profiles of immune cells from control and tumor samples are plotted and colored according to samples; C: Cells are plotted and colored according to cell clusters. Each point corresponds to a single cell colored according to the cell cluster; D: A bubble plot of typical marker genes in each cluster is displayed. Darker colors indicate higher average expression; larger bubbles indicate a greater percentage of genes; E: Histogram of the abundance distribution of each cell subpopulation in the hepatocellular carcinoma and control groups. HCC: Hepatocellular carcinoma; TCR: T-cell receptor; UMAP: Uniform manifold approximation and projections; GZMH: Granzyme H; MT1E: Metallothionein 1E; NK: Natural killer; Treg: Regulatory T cells; DC: Dendritic cell.