C23 ameliorates carbon tetrachloride-induced liver fibrosis in mice

Figure 5 C23 inhibits transforming growth factor beta/Smad3 activation in the fibrotic pathway. A: Heatmap of the transcriptome array comparing the expression of genes involved in the regulation of fibrosis function between carbon tetrachloride (CCl4)-treated and CCl4+C23-treated mouse livers harvested 30 days after the procedure, n = 3; B: Western blotting was used to examine changes in the expression of factors involved in the transforming growth factor beta (TGF-β)/Smad3 signaling pathway during liver fibrosis. β-Actin was used as an internal reference protein; C: The gray value was calculated for p-Smad-3/Smad-3; D: The gray value was calculated for TGF-β/β-actin. ^aP < 0.05, ^bP < 0.01 vs carbon tetrachloride. CCl4: Carbon tetrachloride; TGF-β: Transforming growth factor beta.